With appropriate software and software settings, these primer artefacts can be avoided.

ARTIC primers) are susceptible to primer errors. Importantly: Primer regions in amplicons (e.g. User guide update with some advice how to obtain SARS-CoV-2 mutations from next generation sequencing (NGS) data. We welcome bug reports if you include a very small example file to help us reproduce your bug.ģ. (2) If the VCF file contains two or more lines with the same genomic coordinate but different REF/ALT values, then the character name in the RDF will need to be checked by hand and possibly edited. But you will need to manually check your VCF file and also the resulting RDF file for the following known bugs: (1) Deletions will not be converted correctly and the character is usually omitted entirely from the sequence in the RDF file even though the character is present in the RDF header.

This converter is 'work in progress', and we include it because it may be useful. New Beta-Version of VCF2RDFconverter.exe to convert VCF files to binary and/or multistate RDF files. But please note that we do not have the human resources to assist users to program, test, or debug their software or scripts.Ģ. nucleotide positions) is increased to 35000 to allow users to more easily create SARS-CoV-2 rdf files with their own software or scripts. Due to user wishes, the limit of characters (e.g. Current Release: Network 10.2.0.0, released 2020 December 30ġ.

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